polyethyleneimine (25 kd (Polysciences inc)
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Polyethyleneimine (25 Kd, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyethyleneimine+(25+kd/polyethylenimine++pei/pm39509441-275-13-16
Average 90 stars, based on 1 article reviews
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Transfection:Article Title: High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha Article Snippet: .. For full-length PTPRA, 200 μg PTPRAFL-YFP was introduced into 200 mL of HEK293 freestyle cells (Thermo Fisher Scientific) by Concentration Assay:Article Title: Re-Engineering Therapeutic Anti-Aβ Monoclonal Antibody to Target Amyloid Light Chain Article Snippet: Prior to transfection, cells were centrifuged before being re-suspended at a cell density of 3 × 10 6 cells/mL in 200 mL CD-CHO medium supplemented with 4% ( v / v ) Glutamax (Thermo Fisher, Life Technologies, Carlsbad, CA, USA). .. HC and LC plasmids were diluted with DMEM medium (Thermo Fisher, Life Technologies, Carlsbad, CA, USA) to a concentration of 40 μg/mL (LC:HC = 4:1, w : w ) and mixed with 25 Incubation:Article Title: Re-Engineering Therapeutic Anti-Aβ Monoclonal Antibody to Target Amyloid Light Chain Article Snippet: Prior to transfection, cells were centrifuged before being re-suspended at a cell density of 3 × 10 6 cells/mL in 200 mL CD-CHO medium supplemented with 4% ( v / v ) Glutamax (Thermo Fisher, Life Technologies, Carlsbad, CA, USA). .. HC and LC plasmids were diluted with DMEM medium (Thermo Fisher, Life Technologies, Carlsbad, CA, USA) to a concentration of 40 μg/mL (LC:HC = 4:1, w : w ) and mixed with 25 other:Article Title: Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells Article Snippet: After 20-30 min incubation at room temperature, the transfection reactions were dispersed dropwise over the HEK293T cells. heVLPs were harvested at 48-72 hours post-transfection. heVLP supernatants were filtered using 0.8 μm pore size mixed cellulose esters membrane filters and transferred to polypropylene Beckman ultracentrifuge tubes that are used with the SW28 rotor (Beckman Coulter #326823). Article Title: Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells Article Snippet: After 20-30 min incubation at room temperature, the transfection reactions were dispersed dropwise over the HEK293T cells. heVLPs were harvested at 48-72 hours post-transfection. heVLP supernatants were filtered using 0.8 lam pore size mixed cellulose esters membrane filters and transferred to polypropylene Beckman ultracentrifuge tubes that are used with the SW28 rotor (Beckman Coulter #326823). |
