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Polysciences inc polyethyleneimine (25 kd
Polyethyleneimine (25 Kd, supplied by Polysciences inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polyethyleneimine+(25+kd/polyethylenimine++pei/pm39509441-275-13-16
Average 90 stars, based on 1 article reviews
polyethyleneimine (25 kd - by Bioz Stars, 2026-11
90/100 stars

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Transfection:

Article Title: High Density of N- and O-Glycosylation Shields and Defines the Structural Dynamics of the Intrinsically Disordered Ectodomain of Receptor-type Protein Tyrosine Phosphatase Alpha
Article Snippet: .. For full-length PTPRA, 200 μg PTPRAFL-YFP was introduced into 200 mL of HEK293 freestyle cells (Thermo Fisher Scientific) by transfection with polyethylenimine (PEI) (linear, 25 kD; Polysciences) while 30 μg PTPRAFL-TST was transfected in 30 mL of HEK293 freestyle cells by FreeStyleTM MAX Reagent (Thermo Fisher Scientific). ..

Concentration Assay:

Article Title: Re-Engineering Therapeutic Anti-Aβ Monoclonal Antibody to Target Amyloid Light Chain
Article Snippet: Prior to transfection, cells were centrifuged before being re-suspended at a cell density of 3 × 10 6 cells/mL in 200 mL CD-CHO medium supplemented with 4% ( v / v ) Glutamax (Thermo Fisher, Life Technologies, Carlsbad, CA, USA). .. HC and LC plasmids were diluted with DMEM medium (Thermo Fisher, Life Technologies, Carlsbad, CA, USA) to a concentration of 40 μg/mL (LC:HC = 4:1, w : w ) and mixed with 25 KD polyethyleneimine (PEI; Polysciences, Warrington, PA, USA) (DNA:PEI = 1:2, w : w ) for incubation at room temperature for 15 min. Then, the mixture of DNA and PEI was added to the cell. ..

Incubation:

Article Title: Re-Engineering Therapeutic Anti-Aβ Monoclonal Antibody to Target Amyloid Light Chain
Article Snippet: Prior to transfection, cells were centrifuged before being re-suspended at a cell density of 3 × 10 6 cells/mL in 200 mL CD-CHO medium supplemented with 4% ( v / v ) Glutamax (Thermo Fisher, Life Technologies, Carlsbad, CA, USA). .. HC and LC plasmids were diluted with DMEM medium (Thermo Fisher, Life Technologies, Carlsbad, CA, USA) to a concentration of 40 μg/mL (LC:HC = 4:1, w : w ) and mixed with 25 KD polyethyleneimine (PEI; Polysciences, Warrington, PA, USA) (DNA:PEI = 1:2, w : w ) for incubation at room temperature for 15 min. Then, the mixture of DNA and PEI was added to the cell. ..

other:

Article Title: Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
Article Snippet: After 20-30 min incubation at room temperature, the transfection reactions were dispersed dropwise over the HEK293T cells. heVLPs were harvested at 48-72 hours post-transfection. heVLP supernatants were filtered using 0.8 μm pore size mixed cellulose esters membrane filters and transferred to polypropylene Beckman ultracentrifuge tubes that are used with the SW28 rotor (Beckman Coulter #326823).

Article Title: Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
Article Snippet: After 20-30 min incubation at room temperature, the transfection reactions were dispersed dropwise over the HEK293T cells. heVLPs were harvested at 48-72 hours post-transfection. heVLP supernatants were filtered using 0.8 lam pore size mixed cellulose esters membrane filters and transferred to polypropylene Beckman ultracentrifuge tubes that are used with the SW28 rotor (Beckman Coulter #326823).



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, Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the <t>polyethyleneimine</t> method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.
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, Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the <t>polyethyleneimine</t> method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.
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, Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the <t>polyethyleneimine</t> method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.
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, Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the <t>polyethyleneimine</t> method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.
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, Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the <t>polyethyleneimine</t> method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.
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, Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the <t>polyethyleneimine</t> method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.
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, Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the polyethyleneimine method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.

Journal: PLoS ONE

Article Title: Exploring Dynamics of Molybdate in Living Animal Cells by a Genetically Encoded FRET Nanosensor

doi: 10.1371/journal.pone.0058175

Figure Lengend Snippet: , Time course of R F530:F480 in control cells (for Panel B). HEK-293T was co-transfected with MolyProbe and mock vector by the polyethyleneimine method. Fluorescence was measured after addition of molybdate, and R F530:F480 calculated. B , Time course of Hs MoT2/MFSD5 over-expressing cells. mRNA level of Hs MoT2/MFSD5 was about sixty-fold compared to the control cell. C , Time course of R F530:F480 in control cells (for Panel D) transfected with MolyProbe by X-tremeGENE siRNA reagent. D , Time course of R F530:F480 in Hs MoT2/MFSD5 knockdown cells. mRNA level of Hs MoT2/MFSD5 was 11–35% compared to the control cell. Concentrations of molybdate in working medium are follows: 0 µM (closed circle), 0.1 µM (cross), 0.3 µM (closed triangle), 1 µM (open triangle), 3 µM (closed diamond), 10 µM (open diamond), 30 µM (closed square), 100 µM (open square). Averages and SDs from triplicate samples are shown. n = 3.

Article Snippet: Co-transfection of cells with pYN723 and pYN769 were performed by the polyethyleneimine transfection method using 25 kD linear- polyethyleneimine (Polyscience) , .

Techniques: Transfection, Plasmid Preparation, Fluorescence, Expressing